單細胞示蹤成像技術助力神經發育研究



本期文章:《細胞》:Online/在線發表

美國霍華德休斯醫學研究所Philipp J. Keller和Yinan Wan等研究人員,在整個發育神經回路中利用單細胞示蹤成像技術重構了新興神經元群體的活動。2019年9月26日,《細胞》在線發表了這項成果。

研究人員提出了一種成像方法,用于從神經發生到指示最早的自發運動行為的模式化活動的出現,全面跟蹤整個發育中的斑馬魚脊髓的神經元譜系、運動、分子身份和活動。研究人員發現運動神經元首先是活躍的,并與鄰近的神經元形成局部模式的集成。這些集成合并,達到閾值大小后全局同步,最后招募連合中間神經元以協調對脊椎動物運動很重要的左右交替模式。單個神經元根據其出生時間和解剖學起源定型地進行功能性成熟。

這項研究提供了重構胚胎發生過程中如何發揮功能性回路的通用策略。

研究人員表示,動物的生存需要在胚胎發生過程中發育正常的神經系統。新生神經元必須組裝成產生能夠指示行為的活動模式的回路。要闡明該過程的協調方式,需要采用新方法,這些方法應遵循整個發育回路中所有細胞的成熟和活動。

附:英文原文

Title: Single-Cell Reconstruction of Emerging Population Activity in an Entire Developing Circuit

Author: Yinan Wan, Ziqiang Wei, Loren L. Looger, Minoru Koyama, Shaul Druckmann, Philipp J. Keller

Issue&Volume: 26 September 2019

Abstract: 

Animal survival requires a functioning nervous system to develop during embryogenesis. Newborn neurons must assemble into circuits producing activity patterns capable of instructing behaviors. Elucidating how this process is coordinated requires new methods that follow maturation and activity of all cells across a developing circuit. We present an imaging method for comprehensively tracking neuron lineages, movements, molecular identities, and activity in the entire developing zebrafish spinal cord, from neurogenesis until the emergence of patterned activity instructing the earliest spontaneous motor behavior. We found that motoneurons are active first and form local patterned ensembles with neighboring neurons. These ensembles merge, synchronize globally after reaching a threshold size, and finally recruit commissural interneurons to orchestrate the left-right alternating patterns important for locomotion in vertebrates. Individual neurons undergo functional maturation stereotypically based on their birth time and anatomical origin. Our study provides a general strategy for reconstructing how functioning circuits emerge during embryogenesis.

DOI: 10.1016/j.cell.2019.08.039

Source: https://www.cell.com/cell/fulltext/S0092-8674(19)30958-4

期刊信息

Cell:《細胞》,創刊于1974年。隸屬于細胞出版社,最新IF:36.216

官方網址:https://www.cell.com/

投稿鏈接:https://www.editorialmanager.com/cell/default.aspx




上一篇:上海生科院利用譜系示蹤技術揭示心臟側枝動脈
下一篇:華山醫院運動醫學科陳世益教授課題組研究新成
贵州快3开奖结果